Human LSM5 antibody | NKMAX ATGA0385 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human LSM5 antibody

catalog :

ATGA0385

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT25F8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human LSM5 antibody

Catalog number (SKU) :

ATGA0385

NCBI :

NP_036454

Description :

LSM5, also known as YER146W, is a 91 amino acid protein belonging to the snRNP Sm family. Localized to the nucleus, LSm5 has been found to play a role in the formation and function of the U6 snRNP. Specifically, LSm5 binds to the 3' end of U6 snRNA and facilitates U4/U6 duplex formation. Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Alternative Names :

u6 snRNA-associated Sm-like protein LSm5 isoform a, LSM5 homolog, u6 small nuclear RNA associated (S.cerevisiae), YER146W

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Salgado-Garrido J., et al. (1999) EMBO J. 18: 3451-3462.;; Achsel T., et al. (1999) EMBO J. 18: 5789-5802.;; Friesen WJ., et al. (2000) J Biol Chem. 275: 26370-26375.;; Eystathioy T., et al. (2002) Arthritis Rheum. 46: 726-734.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human LSM5 (1-91aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of LSM5 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LSM5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LSM5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : K562 cell lysate ;;Lane 3. : Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT25F8

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9Y4Y9

Internal Search Terms :

LSM5, u6 snRNA-associated Sm-like protein LSm5 isoform a, LSM5 homolog, u6 small nuclear RNA associated (S.cerevisiae), YER146W,ATGA0385-10ug,ATGA0385-20ug,ATGA0385-50ug,ATGA0385-100ug,ATGA0385-250ug,ATGA0385-500ug,ATGA0385-1mg,ATGA0385-10,ATGA0385-20,ATGA0385-50,ATGA0385-100,ATGA0385-250,ATGA0385-500,ATGA0385-1

more info or order :

NKMAX product webpage