Human S100A4 antibody | NKMAX ATGA0384 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human S100A4 antibody

catalog :

ATGA0384

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1C3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human S100A4 antibody

Catalog number (SKU) :

ATGA0384

NCBI :

NP_002952

Description :

S100 calcium-binding protein A4 (S100A4) is member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Alternative Names :

S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Stoler A., et al. (1985) Proc Natl Acad Sci USA. 82(2): 570-574.;; Choudhary C., et al. (2009) Science. 325: 834-840.;; Bian Y., et al. (2014) J Proteomics. 96: 253-262.;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human S100A4 (1-101aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of S100A4 in A549 cells. The cell was stained with ATGA0384 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of S100A4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining, Wheat germ agglutinin (WGA) with fluorescein conjugate (Red) for plasma membrane staining and monoclonal anti-human S100A4 antibody{2004010323173395.jpg}

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : A549 cell lysate ;;Lane 3. : NIH/3T3 cell lysate ;;Lane 4. : 3T3-L1 cell lysate

Reacts With :

Human

Clone Number :

AT1C3

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cardiovascular

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P26447

Internal Search Terms :

S100A4, S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a,ATGA0384-10ug,ATGA0384-20ug,ATGA0384-50ug,ATGA0384-100ug,ATGA0384-250ug,ATGA0384-500ug,ATGA0384-1mg,ATGA0384-10,ATGA0384-20,ATGA0384-50,ATGA0384-100,ATGA0384-250,ATGA0384-500,ATGA0384-1

more info or order :

NKMAX product webpage