Human PGK1 antibody | NKMAX ATGA0381 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PGK1 antibody

catalog :

ATGA0381

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2F4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PGK1 antibody

Catalog number (SKU) :

ATGA0381

NCBI :

NP_000282

Description :

Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP.

Alternative Names :

Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGKA, MIG10

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Svaasand EK., et al. (2007) Muscle Nerve. 36(5): 679-684. ;; Valentin C., et al. (1998) Hum Mutat. 12(4): 280-287.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PGK1 (1-417aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PGK1 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : LNCaP cell lysate ;;Lane 3. : A549 cell lysate ;;Lane 4. : HepG2 cell lysate ;;Lane 5. : 293T cell lysate ;;Lane 6. : K562 cell lysate ;;Lane 7. : Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT2F4

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00558

Internal Search Terms :

PGK1, Phosphoglycerate kinase 1, Pgk-1,ATGA0381-10ug,ATGA0381-20ug,ATGA0381-50ug,ATGA0381-100ug,ATGA0381-250ug,ATGA0381-500ug,ATGA0381-1mg,ATGA0381-10,ATGA0381-20,ATGA0381-50,ATGA0381-100,ATGA0381-250,ATGA0381-500,ATGA0381-1

more info or order :

NKMAX product webpage