Human Otubain-1/OTUB1 antibody | NKMAX ATGA0380 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Otubain-1/OTUB1 antibody

catalog :

ATGA0380

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT17E10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Otubain-1/OTUB1 antibody

Catalog number (SKU) :

ATGA0380

NCBI :

NP_060140

Description :

Otubain 1, also known as OTUB1, is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system via its interaction with an ubiquitin protease and E3 ubiquitin ligase. This protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth.

Alternative Names :

ubiquitin thioesterase Otubain 1, OTB1, OTu1, ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTuB1, OTu domain containing ubiquitin aldehyde binding protein 1, Otubain 1, ubiquitin specific processing protease OTuB1, ubiquitin thioesterase OTuB1.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Balakirev MY., et al. (2003) EMBO Rep. 4(5): 5175-5122.;; Soares L., et al. (2004) Nat Immunol. 5(1): 45-54.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human OTUB1 (1-271aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of OTUB1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OTUB1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OTUB1 antibody (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : A549 cell lysate ;;Lane 2. : HeLa cell lysate ;;Lane 3. : HepG2 cell lysate ;;Lane 4. : 293T cell lysate

Reacts With :

Human

Clone Number :

AT17E10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q96FW1

Internal Search Terms :

Otubain1, OTUB1, ubiquitin thioesterase Otubain 1, OTB1, OTu1, ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTuB1, OTu domain containing ubiquitin aldehyde binding protein 1, Otubain 1, ubiquitin specific processing protease OTuB1, ubiquitin thioesterase OTuB1.,ATGA0380-10ug,ATGA0380-20ug,ATGA0380-50ug,ATGA0380-100ug,ATGA0380-250ug,ATGA0380-500ug,ATGA0380-1mg,ATGA0380-10,ATGA0380-20,ATGA0380-50,ATGA0380-100,ATGA0380-250,ATGA0380-500,ATGA0380-1

more info or order :

NKMAX product webpage