Human Peroxiredoxin 5/PRDX5 antibody | NKMAX ATGA0379 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Peroxiredoxin 5/PRDX5 antibody

catalog :

ATGA0379

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT6A10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Peroxiredoxin 5/PRDX5 antibody

Catalog number (SKU) :

ATGA0379

NCBI :

NP_036226

Description :

Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. It has been reported that peroxiredoxin 5 is involved in intracellular redox signaling.

Alternative Names :

Alu corepressor 1, Antioxidant enzyme B166, AOEB166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Prx-V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, ACR1, AOEB166, PRXV, SBBI10

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Yamashita H., et al. (1999) J Biol Chem. 274(42): 29897-29904. ;; Declercg JP., et al. (2001) J Mol Biol. 311(4): 751-759.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human Peroxiredoxin 5 (53-214aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of PRDX5 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of PRDX5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0379-IF2.jpg}

WB Comment and Pic :

The Recombinant Human PRDX5 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : Recombinant PRDX5 protein ;;Lane 2. : MCF-7 cell lysate ;;Lane 3. : A549 cell lysate ;;Lane 4. : HeLa cell lysate ;;Lane 5. : 293T cell lysate ;;Lane 6. : Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT6A10

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PRDX5 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Redox Proteins

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P30044

Internal Search Terms :

Peroxiredoxin5, Peroxiredoxin 5, ACR1, AOEB116, B116, PLP, PMP20, PRDX5, PRXV, SBBI10, AOEB166, PRDX 5, Thioredoxin reductase.,ATGA0379-10ug,ATGA0379-20ug,ATGA0379-50ug,ATGA0379-100ug,ATGA0379-250ug,ATGA0379-500ug,ATGA0379-1mg,ATGA0379-10,ATGA0379-20,ATGA0379-50,ATGA0379-100,ATGA0379-250,ATGA0379-500,ATGA0379-1

more info or order :

NKMAX product webpage