Human Dihydrofolate Reductase/DHFR antibody | NKMAX ATGA0376 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Dihydrofolate Reductase/DHFR antibody

catalog :

ATGA0376

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5B2

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Dihydrofolate Reductase/DHFR antibody

Catalog number (SKU) :

ATGA0376

NCBI :

NP_000782

Description :

Dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX) -resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Alternative Names :

Dihydrofolate reductase, EC 1.5.1.3

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Chen MJ., et al. (1984) J Biol Chem. 259(6): 3933-3943. ;; Cody V., et al. (2009) Biochemistry. 48(8): 1702-1711.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human DHFR (1-187aa) purified from E. coli

Applications :

ELISA, WB

IF Comment And Pic :

ICC/IF analysis of DHFR in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DHFR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : Jurkat cell lysate ;;Lane 3 : 293T cell lysate

Reacts With :

Human

Clone Number :

AT5B2

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Other

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00374

Internal Search Terms :

Dihydrofolate Reductase, DHFR, Dihydrofolate reductase, EC 1.5.1.3,ATGA0376-10ug,ATGA0376-20ug,ATGA0376-50ug,ATGA0376-100ug,ATGA0376-250ug,ATGA0376-500ug,ATGA0376-1mg,ATGA0376-10,ATGA0376-20,ATGA0376-50,ATGA0376-100,ATGA0376-250,ATGA0376-500,ATGA0376-1

more info or order :

NKMAX product webpage