Human NUDT1/MTH1 antibody | NKMAX ATGA0375 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NUDT1/MTH1 antibody

catalog :

ATGA0375

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3B3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NUDT1/MTH1 antibody

Catalog number (SKU) :

ATGA0375

NCBI :

NP_945191

Description :

NUDT1, also known as MTH1 is a 179 amino acid cytoplasmic protein that is a member of the nudix hydrolase family. Highly expressed in testis, thymus and proliferating blood lymphocytes, NUDT1 functions as an antimutagenic that hydrolyzes oxidized purine nucleoside triphosphates to their corresponding monophosphates. Through its ability to enzymatically hydrolyze ATP and GTP to AMP and GMP, respectively, NUDT1 prevents misincorporation of GTP into DNA, thus preventing A:T to C:G transversions.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Satoh J., et al. (2000) Eur J Neurol. 7(6): 673-677. ;; Choi KM., et al. (2015) FEBS Lett. 589(3): 349-357.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human NUDT1 (1-156aa) purified from E. coli.

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of NUDT1 in A549 cells. The cell was stained with ATGA0375 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NUDT1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: K562 cell lysate;;Lane 2.: SW480 cell lysate;;Lane 3.: THP-1 cell lysate;;Lane 4.: Jurkat cell lysate;;Lane 5.: A549 cell lysate

Reacts With :

Human

Clone Number :

AT3B3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of NUDT1 in Jurkat cells. The cell was stained with ATGA0375 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P36639

Internal Search Terms :

7,8-dihydro-8-oxoguanine triphosphatase", 78-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21, MTH1, Nudix motif 1, NUDT1, ATGA0375-10ug, ATGA0375-20ug, ATGA0375-50ug, ATGA0375-100ug, ATGA0375-250ug, ATGA0375-500ug, ATGA0375-1mg, ATGA0375-010, ATGA0375-020, ATGA0375-050, ATGA0375-100, ATGA0375-250, ATGA0375-500, ATGA0375-01M

more info or order :

NKMAX product webpage