Human Glycine N-methyltransferase/GNMT antibody | NKMAX ATGA0370 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Glycine N-methyltransferase/GNMT antibody

catalog :

ATGA0370

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5D1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Glycine N-methyltransferase/GNMT antibody

Catalog number (SKU) :

ATGA0370

NCBI :

NP_061833

Description :

Ras-related protein Glycine N-methyltransferase (GNMT) catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Alternative Names :

Glycine N-methyltransferase, GNMT, Glycine N-methyltransferase, Glycine N-methyltransferase EC 2.1.1.20, Glycine N methyltransferase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Velichkova., et al. (2006) J Phys Chem B. 109: 8216-8219.;; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23: 1384.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human GNMT (1-295aa) purified from E. coli.

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of GNMT in HepG2 cells. The cell was stained with ATGA0370 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein (50ng) and tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human GNMT protein;;Lane 2.: Mouse Liver tissue lysate The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: GNMT Transfected 293T cell lysate {ATGA0370-WB2.jpg}

Reacts With :

Human

Clone Number :

AT5D1

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q14749

Internal Search Terms :

Glycine N-methyltransferase, GNMT,ATGA0370-10ug,ATGA0370-20ug,ATGA0370-50ug,ATGA0370-100ug,ATGA0370-250ug,ATGA0370-500ug,ATGA0370-1mg,ATGA0370-10,ATGA0370-20,ATGA0370-50,ATGA0370-100,ATGA0370-250,ATGA0370-500,ATGA0370-1

more info or order :

NKMAX product webpage