Human FUBP1 antibody | NKMAX ATGA0369 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human FUBP1 antibody

catalog :

ATGA0369

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT14F5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human FUBP1 antibody

Catalog number (SKU) :

ATGA0369

NCBI :

NP_003893

Description :

FUBP1 is a transcriptional regulator and fulfills an important function in the precise control of c-myc transcription. The c-Myc protein is a transcription factor which regulates the transcription of many different target genes that play a role in proliferation, cell cycle progression, differentiation, apoptosis and cell metabolism. Consequently, FUBP1 is also involved in the regulation of proliferation and differentiation, as confirmed by different experimental approaches. Knockdown of FUBP1 or expression of a dominant-negative FUBP1 (DNA-binding domain lacking effector activity) led to proliferation arrest in U2OS and Saos-2 osteosarcoma cells due to reduced c-myc expression.

Alternative Names :

Far upstream element (FuSE) binding protein 1, FBP, FuBP

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Duncan R., et al. (1994) Genes Dev. 8(4): 465-480.;; Liu J., et al. (2006) EMBO J. 25(10): 2119-2130.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human FUBP1 (279-448aa) purified from E.coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of FUBP1 in HeLa cells. The cell was stained with ATGA0369 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUBP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: HepG2 cell lysate;;Lane 3.: Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT14F5

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q96AE4

Internal Search Terms :

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more info or order :

NKMAX product webpage