Human Fumarase/FH antibody | NKMAX ATGA0366 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Fumarase/FH antibody

catalog :

ATGA0366

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3F6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Fumarase/FH antibody

Catalog number (SKU) :

ATGA0366

NCBI :

NP_000134

Description :

Fumarase (Fumarate hytdratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid (TCA), or Krebs cycle. This enzyme exists in both a cytosolic form and an N-terminal extended mitochondrial form. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase deficiency can lead to progressive encephalopathy, cerebral atrophy and developmental delay and this enzyme also is thought to act as a tumor suppressor.

Alternative Names :

Fumarate hydratase, FH, HLRCC, LRCC, MCL, MCuL1, Fumarate hydratase Fumarase, Fumarate hydratase mitochondrial, MCuL 1, Multiple hereditary cutaneous leiomyomata.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lehtonen R., et al. (2003) J Med Genet. 40(3): e19. ;; Toro JR., et al. (2003) Am J Hum Genet. 73(1): 95-106.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human Fumarase (44-510aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of Fumarase in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Fumarase antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Fumarase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : LNCap cell lysate ;;Lane 3. : A549 cell lysate ;;Lane 4. : HepG2 cell lysate ;;Lane 5. : 293T cell lysate ;;Lane 6. : K562 cell lysate ;;Lane 7. : Jurkat cell lysate

Reacts With :

Human

Clone Number :

AT3F6

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Fumarase in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P07954

Internal Search Terms :

Fumarase, Fumarate hydratase, FH, HLRCC, LRCC, MCL, MCuL1, Fumarate hydratase Fumarase, Fumarate hydratase mitochondrial, MCuL 1, Multiple hereditary cutaneous leiomyomata.,ATGA0366-10ug,ATGA0366-20ug,ATGA0366-50ug,ATGA0366-100ug,ATGA0366-250ug,ATGA0366-500ug,ATGA0366-1mg,ATGA0366-10,ATGA0366-20,ATGA0366-50,ATGA0366-100,ATGA0366-250,ATGA0366-500,ATGA0366-1

more info or order :

NKMAX product webpage