Human Carbonyl reductase 1/CBR1 antibody | NKMAX ATGA0364 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Carbonyl reductase 1/CBR1 antibody

catalog :

ATGA0364

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2D6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Carbonyl reductase 1/CBR1 antibody

Catalog number (SKU) :

ATGA0364

NCBI :

NP_001748

Description :

Carbonyl reductase 1 (CBR1) is a NADPH-dependent, monomeric, and cytosolic enzyme belonging to a family of short-chain dehydrogenases/reductases. This protein consists of 277 amino acid residues and is widely distributed in human tissues such as liver, epidermis, stomach, small intestine, kidney, neuronal cells, and smooth muscle fiber. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer drug, doxorubicin. The best substrates of CBR1 are quinones, including ubiquinone-1 and tocophrolquinone (vitamin E).

Alternative Names :

Carbonyl reductase 1, Carbonyl reductase 1, CBR, hCBR1, SDR21C1, CBR1, Carbonyl reductase 1 15 hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl reductase [NADPH] 1.CBR 1, CRN, NADPH dependent carbonyl reductase 1, Prostaglandin 9 ketoreductase, Prostaglandin E(2) 9 reductase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lemieux N., et al. (1993) Genomics. 15(1): 169-172.;; Wermuth B., et al. (1986). Biochem Pharmacol. 35 (8): 1277-1282.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human CBR1 (1-277aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of CBR1 in HeLa cells. The cell was stained with ATGA0364 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Recombinant Human CBR1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant Human CBR1;;Lane 2. : HeLa cell lysate;;Lane 3. : 293T cell lysate;;Lane 4. : MCF-7 cell lysate;;Lane 5. : HepG2 cell lysate

Reacts With :

Human

Clone Number :

AT2D6

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CBR1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P16152

Internal Search Terms :

CBR1,ATGA0364-10ug,ATGA0364-20ug,ATGA0364-50ug,ATGA0364-100ug,ATGA0364-250ug,ATGA0364-500ug,ATGA0364-1mg,ATGA0364-10,ATGA0364-20,ATGA0364-50,ATGA0364-100,ATGA0364-250,ATGA0364-500,ATGA0364-1

more info or order :

NKMAX product webpage