Human Cyclin H antibody | NKMAX ATGA0363 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cyclin H antibody

catalog :

ATGA0363

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3G6

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cyclin H antibody

Catalog number (SKU) :

ATGA0363

NCBI :

NP_001230

Description :

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

Alternative Names :

CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jeang KT., et al. (1998) J Biomed Sci. 5(1): 24-27.;; Yankulov K., et al. (1998) Current Biology. 8(13): 447-449.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human Cyclin H (1-323aa) purified from E.coli

Applications :

ELISA,WB

WB Comment and Pic :

The recombinant proteins (25ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human Cyclin H. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HepG2 cell lysate ;;Lane 2.: Jurkat cell lysate ;;Lane 3.: Ramos cell lysate ;;Lane 4.: Balb-3T3 cell lysate {ATGA0363-WB2.jpg}

Reacts With :

Human

Clone Number :

AT3G6

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000.

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P51946

Internal Search Terms :

Cyclin H, CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H,ATGA0363-10ug,ATGA0363-20ug,ATGA0363-50ug,ATGA0363-100ug,ATGA0363-250ug,ATGA0363-500ug,ATGA0363-1mg,ATGA0363-10,ATGA0363-20,ATGA0363-50,ATGA0363-100,ATGA0363-250,ATGA0363-500,ATGA0363-1

more info or order :

NKMAX product webpage