Human Peroxiredoxin 6/PRDX6 antibody | NKMAX ATGA0362 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Peroxiredoxin 6/PRDX6 antibody

catalog :

ATGA0362

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT22E7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Peroxiredoxin 6/PRDX6 antibody

Catalog number (SKU) :

ATGA0362

NCBI :

NP_004896

Description :

Peroxiredoxin 6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell. it can reduce H (2) O (2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Alternative Names :

1-Cys peroxiredoxin, 1-Cys PRX, 24 kDa protein, Acidic calcium-independent phospholipase A2, AiPLA2, Antioxidant protein 2, Glutathione-dependent peroxiredoxin Liver 2D page spot 40, Lysophosphatidylcholine acyltransferase 5, Non-selenium glutathione peroxidase, NSGPx, Red blood cells page spot 12, AOP2, KIAA0106, LPCAT-5

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Phelan SA., et al. (2001) Antioxid Redox Signal. 1(4): 571-584.;; Trevisan R., et al. (2014) Comp Biochem Physiol C Toxicol Phamacol. 159: 22-30.;; McDonagh B., et al. (2014) J Proteome Res. 13: 5008-5021.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PRDX6 (1-224aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of PRDX6 in HeLa cells. The cell was stained with ATGA0362 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant human PRDX6 protein (50ng), Cell and Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 6 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant PRDX6 protein;;Lane 2.: HeLa cell lysate;;Lane 3.: 293T cell lysate;;Lane 4.: HepG2 cell lysate;;Lane 5.: U87MG cell lysate;;Lane 6.: Ramos cell lysate

Reacts With :

Human

Clone Number :

AT22E7

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of Peroxiredoxin in HeLa cells. The cell was stained with ATGA0362 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Redox Proteins

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P30041

Internal Search Terms :

Peroxiredoxin6,ATGA0362-10ug,ATGA0362-20ug,ATGA0362-50ug,ATGA0362-100ug,ATGA0362-250ug,ATGA0362-500ug,ATGA0362-1mg,ATGA0362-10,ATGA0362-20,ATGA0362-50,ATGA0362-100,ATGA0362-250,ATGA0362-500,ATGA0362-1

more info or order :

NKMAX product webpage