Human Fascin/FSCN1 antibody | NKMAX ATGA0361 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Fascin/FSCN1 antibody

catalog :

ATGA0361

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT13D2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Fascin/FSCN1 antibody

Catalog number (SKU) :

ATGA0361

NCBI :

NP_003079

Description :

FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells.

Alternative Names :

Fascin, FAN1, HSN, SNL, Fascin

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wu D., et al. (2010) Ann Oncol. 21(3): 589-596. ;; Shin H., et al. (2009) Phys Rev Left. 103(23): 238102.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human FSCN1 (1-493aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of FSCN1 in HeLa cells. The cell was stained with ATGA0361 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FSCN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : HeLa cell lysate;;Lane 2. : HepG2 cell lysate;;Lane 3. : THP-1 cell lysate

Reacts With :

Human

Clone Number :

AT13D2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of FSCN1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q16658

Internal Search Terms :

Fascin, FSCN1,ATGA0361-10ug,ATGA0361-20ug,ATGA0361-50ug,ATGA0361-100ug,ATGA0361-250ug,ATGA0361-500ug,ATGA0361-1mg,ATGA0361-10,ATGA0361-20,ATGA0361-50,ATGA0361-100,ATGA0361-250,ATGA0361-500,ATGA0361-1

more info or order :

NKMAX product webpage