Human PA28 alpha/PSME1 antibody | NKMAX ATGA0360 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PA28 alpha/PSME1 antibody

catalog :

ATGA0360

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT12H3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PA28 alpha/PSME1 antibody

Catalog number (SKU) :

ATGA0360

NCBI :

NP_006254

Description :

PSME1, also known as PA28 alpha, is an interferon gamma (IFNG) inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. The PSME1 complex is an alternative proteasome activator that does not employ the use of ubiquitin. The PSME1 complex is composed of two homologous subunits, alpha and beta, which have similar catalytic properties and associate to form a hexameric ring.

Alternative Names :

Proteasome activator subunit 1, 11S regulator complex subunit alpha, REG-alpha, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, Proteasome activator 28 subunit alpha, PA28a, PA28alpha, IFI5111

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ahn K., et al. (1996) J Biol Chem. 271(30): 18237-18242. ;; Murata S., et al. (2001) EMBO J. 20(21): 5898-5907.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PSME1 (1-249aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of PSME1 in PC3 cells. The cell was stained with ATGA0360 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : HeLa cell lysate ;;Lane 2. : LNCap cell lysate ;;Lane 3. : A549 cell lysate ;;Lane 4. : 293T cell lysate ;;Lane 5. : Jurkat cell lysate ;;Lane 6. : Raji cell lysate ;;Lane 7. : A431 cell lysate

Reacts With :

Human

Clone Number :

AT12H3

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Ubiquitination

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q06323

Internal Search Terms :

REG-alpha, REGalpha, PSME1, Proteasome activator subunit 1, Proteasome activator complex subunit 1, Proteasome activator 28 subunit alpha, PA28alpha, PA28a, PA28 Activator a Subunit, MGC8628, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111, Activator of multicatalytic protease subunit 1, 11S regulator complex subunit alpha, REG alpha, PA28 alpha, PA28 A, Interferon gamma up regulated I-5111 protein, Interferon gamma up regulated I5111 protein, ATGA0360-10ug, ATGA0360-20ug, ATGA0360-50ug, ATGA0360-100ug, ATGA0360-250ug, ATGA0360-500ug, ATGA0360-1mg, ATGA0360-010, ATGA0360-020, ATGA0360-050, ATGA0360-100, ATGA0360-250, ATGA0360-500, ATGA0360-01M

more info or order :

NKMAX product webpage