antibody

Human alpha-Smooth Muscle Actin/ACTA2 antibody

ATGA0358

50 ul

170 USD

monoclonal

mouse

nonconjugated

AT132D3

western blot, ELISA, immunocytochemistry, FACS

Human alpha-Smooth Muscle Actin/ACTA2 antibody

ATGA0358

NP_001604

Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic beta- and gamma-actin proteins are referred to as non-muscle actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. Actin has major functions. Firstly, F-actin polymers form microfilaments - polar intracellular tracks for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. actin has a role in cell motility through polymerization and depolymerization of fibrils.

Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Herman IM. et al. (1993) Curr Opin Cell Biol. 5(1): 48-55.;; Guo DC., et al. (2009) Am J Hum Genet. 84(5): 617-627.

Mouse

IgG1 kappa

Recombinant human ACTA2 (3-377aa) purified from E. coli

ELISA, WB, ICC/IF, FACS

ICC/IF analysis of ACTA2 in HeLa cells. The cell was stained with ATGA0358 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: SW480 cell lysate;;Lane 3.: HeLa cell lysate

Human

AT132D3

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of ACTA2 in Nb2-11 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Human

Actin & Myosin Related

Unconjugated

Monoclonal

By protein-A affinity chromatography

Monoclonal Antibody

P62736

Aortic smooth muscle actin, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, Aortic smooth muscle, Intermediate form, ACTSA, ACTVS, GIG46, Alpha actin 2, Alpha actin2, Cell growth inhibiting gene 46 protein, ATGA0358-10ug, ATGA0358-20ug, ATGA0358-50ug, ATGA0358-100ug, ATGA0358-250ug, ATGA0358-500ug, ATGA0358-1mg, ATGA0358-010, ATGA0358-020, ATGA0358-050, ATGA0358-100, ATGA0358-250, ATGA0358-500, ATGA0358-01M

Liu X, et al. Human aortic valve interstitial cells obtained from patients with aortic valve stenosis are vascular endothelial growth factor receptor 2 positive and contribute to ectopic calcification. (J Pharmacol Sci. 2021){https://pubmed.ncbi.nlm.nih.gov/33451756/};;Sakata N, et al. Optimal temperature for the long-term culture of adult porcine islets for xenotransplantation. (Front Immunol. 2023){https://pubmed.ncbi.nlm.nih.gov/37901206/};;Men S, et al. Role of CD34 in calcification of human aortic valve interstitial cells from patients with aortic valve stenosis.(J Pharmacol Sci.2024){https://pubmed.ncbi.nlm.nih.gov/39313278/}