Human GOT2 antibody | NKMAX ATGA0357 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GOT2 antibody

catalog :

ATGA0357

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT8B11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GOT2 antibody

Catalog number (SKU) :

ATGA0357

NCBI :

NP_002071

Description :

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. Also, GOT2 is a major participant in the malate-aspartate shuttle, which is a passage from the cytosol to the mitochondria. The two enzymes are homodimeric and show close homology. GOT2 has been seen to have a role in cell proliferation, especially in terms of tumor growth.

Alternative Names :

Aspartate aminotransferase 2 mitochondrial, Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Yang H., et al. (2015) The EMBO journal. 34(8): 1110-1125.;; Guidetti P., et al. (2007) Journal of neurochemistry. 102(1): 103-111.

Abs Host :

Mouse

Isotype :

IgG3 kappa

Immunogen :

Recombinant human GOT2 (30-430aa) purified from E.coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of GOT2 in HeLa cells. The cell was stained with ATGA0357 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant human GOT2 (50ng), cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: GOT2 Recombinant protein;;Lane 2.: Ramos cell lysate;;Lane 3.: 293T cell lysate;;Lane 4.: Liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: HepG2 cell lysate;;Lane 3.: Balb/3T3 cell lysate {ATGA0357-WB2.jpg} The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant Human GOT1;;Lane 2.: Recombinant Human GOT2 {ATGA0357-WB3.jpg}

Reacts With :

Human

Clone Number :

AT8B11

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00505

Internal Search Terms :

GOT2,ATGA0357-10ug,ATGA0357-20ug,ATGA0357-50ug,ATGA0357-100ug,ATGA0357-250ug,ATGA0357-500ug,ATGA0357-1mg,ATGA0357-10,ATGA0357-20,ATGA0357-50,ATGA0357-100,ATGA0357-250,ATGA0357-500,ATGA0357-1

more info or order :

NKMAX product webpage