Human Beclin 1/BECN1 antibody | NKMAX ATGA0355 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Beclin 1/BECN1 antibody

catalog :

ATGA0355

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT45E8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Beclin 1/BECN1 antibody

Catalog number (SKU) :

ATGA0355

NCBI :

NP_003757

Description :

Beclin-1 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 is a mammalian ortholog of the yeast autophagy-related gene (Atg) 6 and BEC-1 in the C. elegans nematode. Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy.

Alternative Names :

Beclin 1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, Beclin 1 autophagy related, ATG6, ATG6 autophagy related 6 homolog, VPS30

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Liang XH., et al. (1998) J Virol. 72(11): 8586-8596.;; Takacs-Vellai K., et al. (2005) Curr Biol. 15(16): 1513-1517.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human BECN (1-450aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of BECN1 in HeLa cells. The cell was stained with ATGA0355 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein (10ng) and cell lysate(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human BECN1 protein;;Lane 2.: Balb/3T3 cell lysate;; The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: BECN1 Transfected 293T cell lysate{ATGA0355-WB2.jpg}

Reacts With :

Human

Clone Number :

AT45E8

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of BECN1 in HeLa cells. The cell was stained with ATGA0355 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q14457

Internal Search Terms :

Beclin1, BECN1,ATGA0355-10ug,ATGA0355-20ug,ATGA0355-50ug,ATGA0355-100ug,ATGA0355-250ug,ATGA0355-500ug,ATGA0355-1mg,ATGA0355-10,ATGA0355-20,ATGA0355-50,ATGA0355-100,ATGA0355-250,ATGA0355-500,ATGA0355-1

more info or order :

NKMAX product webpage