Human ACOT8 antibody | NKMAX ATGA0353 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ACOT8 antibody

catalog :

ATGA0353

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4D10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ACOT8 antibody

Catalog number (SKU) :

ATGA0353

NCBI :

NP_005460

Description :

Acyl-coenzyme A thioesterase 8 is an enzyme that in humans is encoded by the ACOT8 gene. The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene.

Alternative Names :

Acyl-coenzyme A thioesterase 8, hACTE-III, HNAACTE, the, PTE-1, PTE-2, PTE1, PTE2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jones JM., et al. (1999) J Biol Chem. 274(14): 9216-9223.;; Liu LX., et al.(1997) J Biol Chem. 272(21): 13779-13785.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human ACOT8 (1-319aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of ACOT8 in A549 cells. The cell was stained with ATGA0353 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant Human ACOT8 (10ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant Human ACOT8;;Lane 2.: Ramos cell lysate;;Lane 3.: A431 cell lysate;;Lane 4 : 293T cell lysate;;Lane 5 : A549 cell lysate

Reacts With :

Human

Clone Number :

AT4D10

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ACOT8 in CTLL-2 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O14734

Internal Search Terms :

ACOT8,ATGA0353-10ug,ATGA0353-20ug,ATGA0353-50ug,ATGA0353-100ug,ATGA0353-250ug,ATGA0353-500ug,ATGA0353-1mg,ATGA0353-10,ATGA0353-20,ATGA0353-50,ATGA0353-100,ATGA0353-250,ATGA0353-500,ATGA0353-1

more info or order :

NKMAX product webpage