Human PGAM1 antibody | NKMAX ATGA0352 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PGAM1 antibody

catalog :

ATGA0352

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1G4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PGAM1 antibody

Catalog number (SKU) :

ATGA0352

NCBI :

NP_002620

Description :

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X.

Alternative Names :

Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA, Phosphoglycerate mutase 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Vander-Heiden MG., et al. (2010) Science. 329: 1492-1499.;; Jacobowitz DM., et al. (2008) Microvasc Res. 76: 89-93.

Abs Host :

Mouse

Isotype :

IgG2a Lambda

Immunogen :

Recombinant human PGAM1 (1-254aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of PGAM1 in HeLa cells. The cell was stained with ATGA0352 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : 293T cell lysate;;Lane 2. : Jurkat cell lysate;;Lane 3. : Raji cell lysate;;Lane 4. : A431 cell lysate;;Lane 5. : HeLa cell lysate

Reacts With :

Human

Clone Number :

AT1G4

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P18669

Internal Search Terms :

PGAM1,ATGA0352-10ug,ATGA0352-20ug,ATGA0352-50ug,ATGA0352-100ug,ATGA0352-250ug,ATGA0352-500ug,ATGA0352-1mg,ATGA0352-10,ATGA0352-20,ATGA0352-50,ATGA0352-100,ATGA0352-250,ATGA0352-500,ATGA0352-1

more info or order :

NKMAX product webpage