Human PMVK antibody | NKMAX ATGA0349 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PMVK antibody

catalog :

ATGA0349

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT21C10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PMVK antibody

Catalog number (SKU) :

ATGA0349

NCBI :

NP_006547

Description :

Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.

Alternative Names :

Phosphomevalonate kinase, HuMPMKI, PMK, PMKA, PMKASE, Phosphomevalonate kinase

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cho YK., et al. (2001) J Biol chem. 278: 4510-4515.;; Hogenboom S., et al. (2004) J Lipid Res. 45: 697-705.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human PMVK (1-192aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of PMVK in PC3 cells. The cell was stained with ATGA0349 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : HeLa cell lysate;;Lane 2. : HepG2 cell lysate;;Lane 3. : Jukat cell lysate;;Lane 4. : MCF7 cell lysate;;Lane 5. : A549 cell lysate

Reacts With :

Human

Clone Number :

AT21C10

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PMVK in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q15126

Internal Search Terms :

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more info or order :

NKMAX product webpage