Human GRP94/HSP90B1 antibody | NKMAX ATGA0348 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GRP94/HSP90B1 antibody

catalog :

ATGA0348

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT94B9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GRP94/HSP90B1 antibody

Catalog number (SKU) :

ATGA0348

NCBI :

NP_003290

Description :

GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.

Alternative Names :

Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Randow F., et al. (2001) Nat Cell Biol. 3(10): 231.;; Li Z., et al. (2002) Front Biosci. 7: 731-751.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human GRP94 (22-803aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of GRP94 in U87MG cells. The cell was stained with ATGA0348 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Recombinant protein (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human GRP94 ;;Lane 2.: HeLa cell lysate ;;Lane 3.: Jurkat cell lysate ;;Lane 4.: HepG2 cell lysate

Reacts With :

Human

Clone Number :

AT94B9

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of GRP94 in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P14625

Internal Search Terms :

Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GRP9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein, 94 kDa glucose regulated protein, 94kDa glucose regulated protein, 94kDa glucose-regulated protein, ATGA0348-10ug, ATGA0348-20ug, ATGA0348-50ug, ATGA0348-100ug, ATGA0348-250ug, ATGA0348-500ug, ATGA0348-1mg, ATGA0348-010, ATGA0348-020, ATGA0348-050, ATGA0348-100, ATGA0348-250, ATGA0348-500, ATGA0348-01M

more info or order :

NKMAX product webpage