Human EEF1A1 antibody | NKMAX ATGA0347 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human EEF1A1 antibody

catalog :

ATGA0347

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT23C11

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human EEF1A1 antibody

Catalog number (SKU) :

ATGA0347

NCBI :

NP_001393

Description :

Eukaryotic translation elongation factors 1 alpha, eEF1A1 and eEF1A2, are not only translation factors but also pleiotropic proteins that are highly expressed in human tumors, including breast cancer, ovarian cancer, and lung cancer. eEF1A1 modulates cytoskeleton, exhibits chaperone-like activity and also controls cell proliferation and cell death. Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells.

Alternative Names :

Eukaryotic translation elongation factor 1 alpha 1, Leukocyte receptor cluster (LRC) member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, EEF1A, EF1A, LENG7, EE1A1, EF1A1, EF1alpha1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Huang Y., et al. (2012) Zhonqquo Shi Yan Xue Ye Xue Za Zhi. 20(4): 835-841.;; Browne GJ., et al. (2002) Eur J Biochem. 269(22): 5360-5368.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human EEF1A1 (1-462aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of EEF1A1 in A549 cells. The cell was stained with ATGA0347 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EEF1A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: A549 cell lysate ;;Lane 3.: Raji cell lysate ;;Lane 4.: THP-1 cell lysate ;;Lane 5.: MCF-7 cell lysate

Reacts With :

Human

Clone Number :

AT23C11

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P68104

Internal Search Terms :

EEF1A1,ATGA0347-10ug,ATGA0347-20ug,ATGA0347-50ug,ATGA0347-100ug,ATGA0347-250ug,ATGA0347-500ug,ATGA0347-1mg,ATGA0347-10,ATGA0347-20,ATGA0347-50,ATGA0347-100,ATGA0347-250,ATGA0347-500,ATGA0347-1

more info or order :

NKMAX product webpage