Human Glypican 4/GPC4 antibody | NKMAX ATGA0346 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Glypican 4/GPC4 antibody

catalog :

ATGA0346

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT51E3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Glypican 4/GPC4 antibody

Catalog number (SKU) :

ATGA0346

NCBI :

NP_001439

Description :

The Glypican (GPC) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of Glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the initial stage of neural tube closure, suggesting that GPC4 is unlikely to play an essential role in convergent extension in the mouse.

Alternative Names :

Glypican 4, Glypican 4, dJ900E8.1, glypican proteoglycan 4

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ybot-Gonzalez P., et al. (2005) Dev Dyn. 233(3): 1013-1017.;; Karihaloo A., et al. (2004) Mol Cell Biol. 24(19): 8745-8752.;; Ohkawara B., et al. (2003) Development. 130(10): 2129-2138.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human GPC4 (401-529aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of GPC4 in 293T cells. The cell was stained with ATGA0346 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Recombinant portein (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1. : Recombinant Human GPC4 ;;Lane 2. : Ramos cell lysate

Reacts With :

Human

Clone Number :

AT51E3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of GPC4 in 293T cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O75487

Internal Search Terms :

Glypican4, GPC4,ATGA0346-10ug,ATGA0346-20ug,ATGA0346-50ug,ATGA0346-100ug,ATGA0346-250ug,ATGA0346-500ug,ATGA0346-1mg,ATGA0346-10,ATGA0346-20,ATGA0346-50,ATGA0346-100,ATGA0346-250,ATGA0346-500,ATGA0346-1

more info or order :

NKMAX product webpage