Human Aldolase A/ALDOA antibody | NKMAX ATGA0341 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Aldolase A/ALDOA antibody

catalog :

ATGA0341

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3F9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Aldolase A/ALDOA antibody

Catalog number (SKU) :

ATGA0341

NCBI :

NP_908930

Description :

Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney, and intestine and similar to aldolase C levels in brain and other nervous tissue.

Alternative Names :

Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDA

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Castaldo G., et al.(2000) Clin Chem. 46(7): 901-906.;; Long F., et al.(2014) Oncol Rep. 32(5): 2031-2037.;; Caspi M., et al.(2014) Mol Cancer. 13: 164.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human ALDOA (1-364aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of ALDOA in A549 cells. The cell was stained with ATGA0341 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Recombinant protein and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human ALDOA ;;Lane 2.: HeLa cell lysate ;;Lane 3.: HepG2 cell lysate

Reacts With :

Human

Clone Number :

AT3F9

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ALDOA in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P04075

Internal Search Terms :

ALDOA,ATGA0341-10ug,ATGA0341-20ug,ATGA0341-50ug,ATGA0341-100ug,ATGA0341-250ug,ATGA0341-500ug,ATGA0341-1mg,ATGA0341-10,ATGA0341-20,ATGA0341-50,ATGA0341-100,ATGA0341-250,ATGA0341-500,ATGA0341-1

more info or order :

NKMAX product webpage