Human Cytokeratin 17/KRT17 antibody | NKMAX ATGA0340 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cytokeratin 17/KRT17 antibody

catalog :

ATGA0340

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT9F3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cytokeratin 17/KRT17 antibody

Catalog number (SKU) :

ATGA0340

NCBI :

NP_000413

Description :

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling. Keratinocytes deficient in keratin 17 exhibit abnormal Akt/mTOR signaling and fail to produce an increase in translation, cell size, or growth; these cells also exhibit abnormal 14-3-3 sigma localization.

Alternative Names :

Keratin type I cytoskeletal 17, K17, PC, PC2, PCHC1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kim S., et al. (2006) Nature. 441(7091): 362-365.;; Troyanovsky S.M., et al. (1992) Eur J Cell Biol. 59(1): 127-137.;; McGowan K.M. and Coulombe P.A. (1998) J Cell Biol. 143(2): 469-486.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human KRT17(1-432aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of KRT17 in HeLa cells. The cell was stained with ATGA0340 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of KRT17 in A431 cells. The cell was stained with ATGA0340 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0340-IF2.jpg}

WB Comment and Pic :

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT17 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Recombinant Human KRT17;;Lane 2.: HeLa cell lysate;;Lane 3.: A431 cell lysate

Reacts With :

Human

Clone Number :

AT9F3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of KRT17 in A431 cells. The cell was stained with ATGA0340 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q04695

Internal Search Terms :

K17, Keratin, Keratin type I cytoskeletal 17, KRT17, PC, PC2, PCHC1, type I cytoskeletal 17, ATGA0340-10ug, ATGA0340-20ug, ATGA0340-50ug, ATGA0340-100ug, ATGA0340-250ug, ATGA0340-500ug, ATGA0340-1mg, ATGA0340-010, ATGA0340-020, ATGA0340-050, ATGA0340-100, ATGA0340-250, ATGA0340-500, ATGA0340-01M

more info or order :

NKMAX product webpage