Human CD14 antibody | NKMAX ATGA0339 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CD14 antibody

catalog :

ATGA0339

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT87H7

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CD14 antibody

Catalog number (SKU) :

ATGA0339

NCBI :

NP_001167576

Description :

The protein encoded by this gene is a component of the innate immune system. CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48kDa) or is directly secreted from intracellular vesicles (56kDa). The x-ray crystal structure of human CD14 reveals a monomeric, bent solenoid structure containing a hydrophobic amino-terminal pocket. CD14 was the first described pattern recognition receptor. CD14 acts as a co-receptor (along with the Toll-like receptor TLR4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns such as lipoteichoic acid.

Alternative Names :

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14 molecule

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kirkland T.N., et al. (1998) Prog Clin Biol Res. 397: 79-87.;; Kelley S.L., et al. (2013) Journal of Immunology. 190(3): 1304-1311.;; Kitchens R.L., et al. (2000) Chem Immunol Chemical Immunology and Allergy. 74: 61-82.;; Tapping R.I., et al. (2000) Chem Immunol Chemical Immunology and Allergy. 74: 108-121.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CD14 (20-349aa) purified from E.coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD14 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: A549 cell lysate

Reacts With :

Human

Clone Number :

AT87H7

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CD14 in THP-1 cells. The cell was stained with ATGA0339 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Flow cytometry analysis of CD14 in PBMC cells. The cell was stained with ATGA0339 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black). {ATGA0339-FC2.jpg}

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P08571

Internal Search Terms :

CD14,ATGA0339-10ug,ATGA0339-20ug,ATGA0339-50ug,ATGA0339-100ug,ATGA0339-250ug,ATGA0339-500ug,ATGA0339-1mg,ATGA0339-10,ATGA0339-20,ATGA0339-50,ATGA0339-100,ATGA0339-250,ATGA0339-500,ATGA0339-1

more info or order :

NKMAX product webpage