Human Keratin 14/KRT14 antibody | NKMAX ATGA0338 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Keratin 14/KRT14 antibody

catalog :

ATGA0338

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT13F4

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Keratin 14/KRT14 antibody

Catalog number (SKU) :

ATGA0338

NCBI :

AAH19097

Description :

Keratin 14 (KRT14) is a member of the type I keratin family of intermediate filament proteins. KRT14 gene provides instructions for making a protein called keratin 14. Keratins are a group of tough, fibrous proteins that form the structural framework of certain cells, particularly cells that make up the skin, hair, and nails. Keratin 14 is specifically produced in cells called keratinocytes in the outer layer of the skin.

Alternative Names :

Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Pfendner E.G., et al. (2005) J Invest Dermatol. 125(2): 239-243.;; Arin M.J., et al (2010) Br J Dermatol. 162(6): 1365-1369.;; Muller F.B., et al (2006) Hum Mutat. 27(7): 719-720.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human KRT14 (1-472aa) purified from E. coli.

Applications :

ELISA, WB, ICC/IF, IHC, FACS

IF Comment And Pic :

ICC/IF analysis of KRT14 in PC3 cells. The cell was stained with ATGA0338 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein (50ng) and cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human KRT14 ;;Lane 2.: A431 cell lysate

Reacts With :

Human

Clone Number :

AT13F4

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of KRT14 in A431 cells. The cell was stained with ATGA0338 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

IHC Comment And Pic :

Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT14 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Antigen Species :

Human

Gene Category :

Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P02533

Internal Search Terms :

Cytokeratin14,ATGA0338-10ug,ATGA0338-20ug,ATGA0338-50ug,ATGA0338-100ug,ATGA0338-250ug,ATGA0338-500ug,ATGA0338-1mg,ATGA0338-10,ATGA0338-20,ATGA0338-50,ATGA0338-100,ATGA0338-250,ATGA0338-500,ATGA0338-1

more info or order :

NKMAX product webpage