Human Cytokeratin 20/KRT20 antibody | NKMAX ATGA0337 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Cytokeratin 20/KRT20 antibody

catalog :

ATGA0337

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT10D8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Cytokeratin 20/KRT20 antibody

Catalog number (SKU) :

ATGA0337

NCBI :

NP_061883

Description :

Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor.

Alternative Names :

CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Moll R., et al. (1990) J Cell Biol. 111(2): 567-580.;; Piero D., et al. (2011) Nat Biotechnol. 29(12): 1120-1127.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human KRT20 (1-424aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of KRT20 in A549 cells. The cell was stained with ATGA0337 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The Recombinant protein (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human KRT20 ;;Lane 2.: HeLa cell lysate ;;Lane 3.: A431 cell lysate

Reacts With :

Human

Clone Number :

AT10D8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cancer

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P35900

Internal Search Terms :

KRT20, Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20, CK-20, CK20, K20, KRT21, ATGA0337-10ug, ATGA0337-20ug, ATGA0337-50ug, ATGA0337-100ug, ATGA0337-250ug, ATGA0337-500ug, ATGA0337-1mg, ATGA0337-010, ATGA0337-020, ATGA0337-050, ATGA0337-100, ATGA0337-250, ATGA0337-500, ATGA0337-01M

more info or order :

NKMAX product webpage