Human EDAR antibody | NKMAX ATGA0334 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human EDAR antibody

catalog :

ATGA0334

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT19E8

reactivity :

human

application :

western blot, ELISA, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human EDAR antibody

Catalog number (SKU) :

ATGA0334

NCBI :

NP_071731

Description :

Tumor necrosis factor receptor superfamily member EDAR is a protein that in humans is encoded by the EDAR gene. EDAR and other genes provide instructions for making proteins that work together during embryonic development. These proteins form part of a signaling pathway that is critical for the interaction between two cell layers, the ectoderm and the mesoderm. In the early embryo, these cell layers form the basis for many of the body's organs and tissues. Ectoderm-mesoderm interactions are essential for the proper formation of several structures that arise from the ectoderm, including the skin, hair, nails, teeth, and sweat glands.

Alternative Names :

Tumor necrosis factor receptor superfamily member EDAR, DL, ECTD10A, ECTD10B, ED1R, ED3, ED5, EDA-A1R, EDA1R, EDA3, HRM1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Monreal AW, et al. (1999) Nat Genet 22(4):366-9.;; Aswegan AL, et al. (1997) Am J Med Genet 72(4):462-7.;; Entrez Gene: EDAR ectodysplasin A receptor.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human EDAR (27-448aa) purified from E.coli

Applications :

ELISA, WB, FACS

WB Comment and Pic :

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EDAR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1 : Recombinant protein EDAR ;;Lane 2 : A549 cell lysate ;;Lane 3 : Ramos cell lysate

Reacts With :

Human

Clone Number :

AT19E8

Ab Usage :

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of EDAR in HeLa cells. The cell was stained with ATGA0334 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9UNE0

Internal Search Terms :

EDAR,ATGA0334-10ug,ATGA0334-20ug,ATGA0334-50ug,ATGA0334-100ug,ATGA0334-250ug,ATGA0334-500ug,ATGA0334-1mg,ATGA0334-10,ATGA0334-20,ATGA0334-50,ATGA0334-100,ATGA0334-250,ATGA0334-500,ATGA0334-1

more info or order :

NKMAX product webpage