Human PDCL3 antibody | NKMAX ATGA0333 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PDCL3 antibody

catalog :

ATGA0333

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT8F9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PDCL3 antibody

Catalog number (SKU) :

ATGA0333

NCBI :

NP_076970

Description :

PDCL3 (phosducin-like 3), also known as VIAF1 (viral IAP-associated factor 1), VIAF, PHLP3 or HTPHLP, is a widely expressed protein that belongs to the phosducin-like family of proteins. phosducin-like family members associate with the cytosolic chaperonin complex (CCT) and regulate the folding of proteins. Localizing to the cytoplasm, PDCL3 does not appear to play a role in G protein signaling, but participates in the chaperone-assisted folding of proteins, such as beta Tubulin and Actin, that are involved in the regulation of cell cycle progression. More specifically, PDCL3, when associated with CCT, represses the ATPase activity of CCT and ultimately disrupts the folding of the Actin or tubulin substrates.

Alternative Names :

Phosducin-Like protein 3, Phosducin-Like protein 3, HTPHLP, MGC3062, PHLP2A, PHLP3, VIAF, VIAF1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wilkinson J.C., et al. (2004) Biol. Chem. 279(49):51091-51099.;; Hillier L.W., et al. (2005) Nature 434(7034):724-731.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PDCL3 (1-239aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of PDCL3 in HeLa cells. The cell was stained with ATGA0333 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of PDCL3 in PC3 cells. The cell was stained with ATGA0333 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0333-IF2.jpg}

WB Comment and Pic :

The cell lysates of Jurkat (40ug), A549(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Jurkat cell lysate ;; Lane 2.: A549 cell lysateThe Recombinant protein (100ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Protein PDCL3 100ng{ATGA0333-WB2.jpg}

Reacts With :

Human

Clone Number :

AT8F9

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9H2J4

Internal Search Terms :

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more info or order :

NKMAX product webpage