Human CD3 epsilon/CD3E antibody | NKMAX ATGA0331 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CD3 epsilon/CD3E antibody

catalog :

ATGA0331

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3H3

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CD3 epsilon/CD3E antibody

Catalog number (SKU) :

ATGA0331

NCBI :

NP_000724

Description :

The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multi subunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

Alternative Names :

T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Gold DP., et al. (1986) Nature 321(6068): 431-434.;; Soudais C., et al. (1993) Nat Genet 3(1):77-81.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human CD3E (23-126aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of CD3E in Jurkat cells. The cell was stained with ATGA0331 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Recombinant CD3E proteins (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0331-WB2.jpg}

Reacts With :

Human

Clone Number :

AT3H3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of CD3E in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P07766

Internal Search Terms :

CD3 epsilon, CD3E,ATGA0331-10ug,ATGA0331-20ug,ATGA0331-50ug,ATGA0331-100ug,ATGA0331-250ug,ATGA0331-500ug,ATGA0331-1mg,ATGA0331-10,ATGA0331-20,ATGA0331-50,ATGA0331-100,ATGA0331-250,ATGA0331-500,ATGA0331-1

more info or order :

NKMAX product webpage