Human Aminoacylase/ACY1 antibody | NKMAX ATGA0329 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Aminoacylase/ACY1 antibody

catalog :

ATGA0329

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1E2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Aminoacylase/ACY1 antibody

Catalog number (SKU) :

ATGA0329

NCBI :

NP_000657

Description :

Aminoacylase-1, also designated N-acyl-L-amino-acid amidohydrolase or ACY1, is a member of the largest metallopeptidase family called M20A. Aminoacylase-1 is a zinc-binding homodimeric enzyme expressed in kidney, brain, placenta and spleen. It is the most abundant of the aminoacylases. Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids.

Alternative Names :

Aminoacylase 1, ACY1D, ACYLASE, Aminoacylase 1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cook R.M., et al. (1993) J Biol Chem 268(23): 17010-17017.;; Lindner H.A., et al. (2003) J Biol Chem 278(45): 44496-44504.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human ACY1 (1-408aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of ACY1 in HeLa cells. The cell was stained with ATGA0329 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates of Jurkat, HepG2, Hela, A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACY1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Recombinant ACY1 protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACY1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0329-WB2.jpg}

Reacts With :

Human

Clone Number :

AT1E2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ACY1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q03154

Internal Search Terms :

Aminoacylase, ACY1,ATGA0329-10ug,ATGA0329-20ug,ATGA0329-50ug,ATGA0329-100ug,ATGA0329-250ug,ATGA0329-500ug,ATGA0329-1mg,ATGA0329-10,ATGA0329-20,ATGA0329-50,ATGA0329-100,ATGA0329-250,ATGA0329-500,ATGA0329-1

more info or order :

NKMAX product webpage