Human CRADD antibody | NKMAX ATGA0327 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CRADD antibody

catalog :

ATGA0327

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT14G8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CRADD antibody

Catalog number (SKU) :

ATGA0327

NCBI :

NP_003796

Description :

CRADD, also referred as RAIDD, is an adaptor protein that could interact with both caspase 2 and RIP that can promote apoptosis once activated. RAIDD (RIP-associated ICH-1/Ced-3 homologous protein with a death domain) has been identified as a RIP binding protein that also associates with members of the caspase family, providing a link between activation of the TNF-Rs and the triggering of the cysteine protease cascade. The amino-terminal domain of RAIDD shares significant homology with the prodomain of ICH-1 and mediates the binding of RAIDD to this cysteine protease.

Alternative Names :

Caspase and RIP adapter with death domain, RAIDD, CRADD, Caspase and RIP adapter with death domain, Caspase and RIP adapter with death domain CASP2 and RIPK1 domain containing adaptor with death domain, Death adaptor molecule RAIDD, Death domain containing protein CRADD, MGC9163, RIP associated ICH1/CED3 homologous protein with death domain, RIP associated protein with a death domain.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lin. Q., et al. (2012) J Immunol 188(6): 2493-2497.;; Shen. Q., et al. (2014) Am J Transl Res 6(5): 538-547.;; Ahmad. M., et al. (1997) Cancer Res 57(4): 615-619.

Abs Host :

Mouse

Isotype :

IgG1a kappa

Immunogen :

Recombinant human CRADD (1-199aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of CRADD in HeLa cells. The cell was stained with ATGA0327 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of CRADD in PC3 cells. The cell was stained with ATGA0327 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0327-IF2.jpg}

WB Comment and Pic :

The cell lysates of A549, HeLa, MCF-7 and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: A549 cell lysate ;;Lane 2.: HeLa cell lysate ;;Lane 3.: MCF-7 cell lysate ;;Lane 4.: 293T cell lysateThe Recombinant Human CRADD (50,100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRADD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Protein 50 ng ;;Lane 2.: Recombinant Protein 100 ng{ATGA0327-WB2.jpg}

Reacts With :

Human

Clone Number :

AT14G8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P78560

Internal Search Terms :

CRADD,ATGA0327-10ug,ATGA0327-20ug,ATGA0327-50ug,ATGA0327-100ug,ATGA0327-250ug,ATGA0327-500ug,ATGA0327-1mg,ATGA0327-10,ATGA0327-20,ATGA0327-50,ATGA0327-100,ATGA0327-250,ATGA0327-500,ATGA0327-1

more info or order :

NKMAX product webpage