Human CD64/FCGR1A antibody | NKMAX ATGA0324 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CD64/FCGR1A antibody

catalog :

ATGA0324

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT37F7

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CD64/FCGR1A antibody

Catalog number (SKU) :

ATGA0324

NCBI :

NP_000557

Description :

High affinity immunoglobulin gamma Fc receptor I (FCGR1A) is a protein that in humans is encoded by the FCGR1A gene. Also, This protein is known as CD64, belongs to the immunoglobulin superfamily. It is a high-affinity Fc-gamma receptor. They are playing a role in both innate and adaptive immune responses. They have been shown to interact with FCAR. The FcgRI binds the Fc portion of IgG and causes activation of the host cell via an intercellular ITAM motif.

Alternative Names :

High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, RIA, FcgammaRIa, fc-gamma RIA, CD64, FCGR1A, FCG1, FCGR1, IGFR1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Maresco. D.L., et al. (1996) Cytogenet Cell Genet 73(3): 157-163.;; Maresco. D.L., et al. (1998) Cytogenet Cell Genet 82(1-2): 71-74.;; Morton., et al. (1995) J Biol Chem 270(50): 29781-29787.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human FCGR1A (16-292aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of FCGR1A in HeLa cells. The cell was stained with ATGA0324 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). ICC/IF analysis of FCGR1A in Jurkat cells. The cell was stained with ATGA0324 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010267583857.jpg}

WB Comment and Pic :

The recombinant protein (20ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FCGR1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT37F7

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of FCGR1A in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Flow cytometry analysis of FCGR1A in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). {2004010267727240.jpg}

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P12314

Internal Search Terms :

Fc g R1, CD64, High affinity immunoglobulin gamma Fc receptor I, CD64, CD64A, FCRI, IGFR1,ATGA0324-10ug,ATGA0324-20ug,ATGA0324-50ug,ATGA0324-100ug,ATGA0324-250ug,ATGA0324-500ug,ATGA0324-1mg,ATGA0324-10,ATGA0324-20,ATGA0324-50,ATGA0324-100,ATGA0324-250,ATGA0324-500,ATGA0324-1, Fc gamma RIa

more info or order :

NKMAX product webpage