Human Glutathione Reductase/GSR antibody | NKMAX ATGA0323 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Glutathione Reductase/GSR antibody

catalog :

ATGA0323

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT11D10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Glutathione Reductase/GSR antibody

Catalog number (SKU) :

ATGA0323

NCBI :

NP_000628

Description :

Glutathione reductase (GR) also known as glutathione-disulfide reductase (GSR) is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. Glutathione reductase functions as dimeric disulfide oxidoreductase and utilizes an FAD prosthetic group and NADPH to reduce one mole of GSSG to two moles of GSH.

Alternative Names :

Glutathione reductase, GLuR, GRD1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Deponte M. (2013) Biochim Biophys Acta 1830(5): 3217-3266;; Meister A. (1988) J Biol Chem 263(33): 17205-17208;; Mannervik B. (1987) Biochem Soc Trans 15(4): 717-718

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human GSR (43-522aa) purified from E.coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of GSR in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Recombinant human GSR protein;;

Reacts With :

Human

Clone Number :

AT11D10

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of GSR in Jurkat cells. The cell was stained with ATGA0323 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Redox Proteins

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P00390

Internal Search Terms :

Glutathione Reductase, GSR, Glutathione reductase, GLuR, GRD1,ATGA0323-10ug,ATGA0323-20ug,ATGA0323-50ug,ATGA0323-100ug,ATGA0323-250ug,ATGA0323-500ug,ATGA0323-1mg,ATGA0323-10,ATGA0323-20,ATGA0323-50,ATGA0323-100,ATGA0323-250,ATGA0323-500,ATGA0323-1

more info or order :

NKMAX product webpage