Human NKp44/NCR2 antibody | NKMAX ATGA0322 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human NKp44/NCR2 antibody

catalog :

ATGA0322

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1G6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human NKp44/NCR2 antibody

Catalog number (SKU) :

ATGA0322

NCBI :

NP_004819

Description :

NKp44 (NCR2) is a member of the natural cytotoxicity receptor (NCR) family that is expressed on activated human NK cells. NKp44 displays a single extracellular Ig-like V domain and a transmembrane portion containing the charged residue (Lysine), likely involved in the association with KARAP/DAP12 molecules.

Alternative Names :

Natural cytotoxicity triggering receptor 2 isoform 1, CD336, LY95, NK-p44 NKP44, ANK0501

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Biassoni. R., et al., (2003) J Cell Mol Med 7: 376-387.;; Kerry. S.C., et al., (2004) Journal of Immunology 172: 899-906.

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human NKp44 (19-130aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of NKp44/NCR2 in Jurkat cells. The cell was stained with ATGA0322 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp44/NCR2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human NKp30 protein;;Lane 2.: Recombinant human NKp44 protein;;Lane 3.: Recombinant human NKp46 protein;;

Reacts With :

Human

Clone Number :

AT1G6

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of NKp44/NCR2 in Jurkat cells. The cell was stained with ATGA0322 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Immunology

Additional Comment Only :

ANK0501 has been replaced with a catalog number ATGA0322.

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O95944

Internal Search Terms :

NKp44, NCR2, Natural cytotoxicity triggering receptor 2 isoform 1, CD336, LY95, NK-p44 NKP44,ATGA0322-10ug,ATGA0322-20ug,ATGA0322-50ug,ATGA0322-100ug,ATGA0322-250ug,ATGA0322-500ug,ATGA0322-1mg,ATGA0322-10,ATGA0322-20,ATGA0322-50,ATGA0322-100,ATGA0322-250,ATGA0322-500,ATGA0322-1

more info or order :

NKMAX product webpage