Human LRPAP antibody | NKMAX ATGA0321 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human LRPAP antibody

catalog :

ATGA0321

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT8G8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human LRPAP antibody

Catalog number (SKU) :

ATGA0321

NCBI :

NP_002328

Description :

Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. LRPAP1 is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. LRPAP1 is present on the cell surface, where it is an effective inhibitor of ligand binding to the kidney glycoprotein 330 (gp330) yet it is most abundant in the endoplasmic reticulum lumen and may function in receptor folding and/or trafficking.

Alternative Names :

alpha-2-macroglobulin receptor-associated protein, A2MRAP, A2RAP, HBP44, MGC138272, MRAP, RAP

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kounnas. M.Z., et al. (1992) J Biol Chem 267(29):21162-21166.;; Bu. G., et al. (1995) EMBO J 14(10):2269-2280.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human LRPAP1 (35-357aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of LRPAP in HeLa cells. The cell was stained with ATGA0321 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LRPAP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse kidney tissue lysate

Reacts With :

Human

Clone Number :

AT8G8

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P30533

Internal Search Terms :

LRPAP,ATGA0321-10ug,ATGA0321-20ug,ATGA0321-50ug,ATGA0321-100ug,ATGA0321-250ug,ATGA0321-500ug,ATGA0321-1mg,ATGA0321-10,ATGA0321-20,ATGA0321-50,ATGA0321-100,ATGA0321-250,ATGA0321-500,ATGA0321-1

more info or order :

NKMAX product webpage