Human GRP78/HSPA5 antibody | NKMAX ATGA0320 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GRP78/HSPA5 antibody

catalog :

ATGA0320

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3D2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GRP78/HSPA5 antibody

Catalog number (SKU) :

ATGA0320

NCBI :

NP_005338

Description :

Binding immunoglobulin protein (BIP) also known as 78kDa glucose-regulated protein (GRP-78) or heat shock 70kDa protein 5 (HSPA5) is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BIP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. BIP is an abundant protein under all growth conditions, but its synthesis is markedly induced under conditions that lead to the accumulation of unfolded polypeptides in the ER.

Alternative Names :

Heat shock protein family A member 5, Heat shock 70kD protein 5, HSP70 family protein 5, Glucose-regulated protein 78kD, Binding-immunoglobulin protein, BiP, Endoplasmic reticulum chaperone BiP, Glucose-regulated protein 78kDa, Immunoglobulin heavy chain-binding protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ting. J., et al. (1988) DNA 7(4): 275-86.;; Hendershot. L.M., et al. (1994) Genomics 20(2): 281-284.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human BIP (20-650aa) purified from Baculo virus

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of GRP78/HSPA5 in HeLa cells. The cell was stained with ATGA0320 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of GRP78/HSPA5 in Hep3B cells. The cell was stained with ATGA0320 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0320-IF2.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP78/HSPA5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: MCF7 cell lysate;;

Reacts With :

Human

Clone Number :

AT3D2

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P11021

Internal Search Terms :

GRP78, HSPA5, Binding immunoglobulin protein, HSPA5, heat shock 70kDa protein 5, GRP78, glucose-regulated protein 78kD, MIF2,ATGA0320-10ug,ATGA0320-20ug,ATGA0320-50ug,ATGA0320-100ug,ATGA0320-250ug,ATGA0320-500ug,ATGA0320-1mg,ATGA0320-10,ATGA0320-20,ATGA0320-50,ATGA0320-100,ATGA0320-250,ATGA0320-500,ATGA0320-1

more info or order :

NKMAX product webpage