Human Bub3 antibody | NKMAX ATGA0319 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human Bub3 antibody

catalog :

ATGA0319

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2H6

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human Bub3 antibody

Catalog number (SKU) :

ATGA0319

NCBI :

NP_004716

Description :

BUB3 (budding uninhibited by benzimidazoles 3 homolog) is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments.

Alternative Names :

Mitotic checkpoint protein BuB3, BuB3L, hBuB3

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Loqarinho. E., et al. (2008) Mol Biol Cell 19(4): 1798-1813;; Warren. C.D., et al. (2002) Mol Biol Cell 13(9): 3029-3041;; Taylor. S.S., et al. (1998) J Cell Biol 142(1): 1-11

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human BUB3 (1-328aa) purified from E. coli

Applications :

ELISA,WB

WB Comment and Pic :

The cell lysates of HeLa(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT2H6

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O43684

Internal Search Terms :

Bub3, Mitotic checkpoint protein BuB3, BuB3L, hBuB3,ATGA0319-10ug,ATGA0319-20ug,ATGA0319-50ug,ATGA0319-100ug,ATGA0319-250ug,ATGA0319-500ug,ATGA0319-1mg,ATGA0319-10,ATGA0319-20,ATGA0319-50,ATGA0319-100,ATGA0319-250,ATGA0319-500,ATGA0319-1

more info or order :

NKMAX product webpage