Human GARS antibody | NKMAX ATGA0318 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human GARS antibody

catalog :

ATGA0318

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4E10

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human GARS antibody

Catalog number (SKU) :

ATGA0318

NCBI :

NP_002038

Description :

GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetase that charge tRNAs with their cognate amino acids. Defects in the gene encoding GlyRS is the cause of Charcot-Marie-Tooth disease type 2D (CMT2D), which is an autosomal dominant inherited disease characterized by severe weakness, atrophy and absence of deep tendon reflexes in the upper extremities. Defects in the GlyRS gene is also the cause of distal hereditary muscular neuropathy type V (HMN5), a disease similar to CMT2D, though the distal sensory involvement is less severe in HMN5 patients.

Alternative Names :

Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Xie. W. (2007) Proc Natl Acad Sci U S A. 104: 9976-9981.;; Antonellis. A. (2003) AM J Hum Genet 72: 1293-1299.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human GARS (43-289aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of GARS in HeLa cells. The cell was stained with ATGA0318 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GARS (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: 293T cell lysate;;Lane 3.: Jurkat cell lysate;;Lane 4.: U87-MG cell lysate;;Lane 5.: A431 cell lysate;;

Reacts With :

Human

Clone Number :

AT4E10

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P41250

Internal Search Terms :

GARS,ATGA0318-10ug,ATGA0318-20ug,ATGA0318-50ug,ATGA0318-100ug,ATGA0318-250ug,ATGA0318-500ug,ATGA0318-1mg,ATGA0318-10,ATGA0318-20,ATGA0318-50,ATGA0318-100,ATGA0318-250,ATGA0318-500,ATGA0318-1

more info or order :

NKMAX product webpage