Human CACYBP antibody | NKMAX ATGA0316 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CACYBP antibody

catalog :

ATGA0316

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3G8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CACYBP antibody

Catalog number (SKU) :

ATGA0316

NCBI :

NP_001007215

Description :

CACYBP (Calcyclin-binding protein) is primarily a nuclear protein that contains one CS domain and one SGS domain. CACYBP is believed to be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It most likely serves as a molecular bridge in ubiquitin E3 complexes. It also participates in the ubiquitin-mediated degradation of b-catenin. CACYBP is thought to be a potential inhibitor of cell growth and invasion in the gastric cancer cell through its effects on b-catenin protein expression and transcriptional activation of TCF/LEF.

Alternative Names :

Clcyclin-binding protein, GIG5, PNAS-107, RP1-102G20.6, S100A6BP, SIP, Clcyclin-binding protein CACYBP, Calcyclin binding protein, GIG 5, Growth inhibiting gene 5, Growth inhibiting gene 5 protein, hCacyBP, MGC87971, PNAS 107, PNAS107, RP1 102G20.6, S100A6 binding protein.

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ghosh. D., et al. (2013) Mol Cell Proteomics 12(7): 1865-1880;; Rines. A.K., et al. (2012) FASEB 26(11): 4685-4695;; Ning. X., et al. (2007) Mol Cancer Res 5(12): 1254-1262

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human CACYBP (1-185aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of CACYBP in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CACYBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0316-IF1b.jpg}

WB Comment and Pic :

The cell lysates of HeLa, 293T, Jurkat and A549(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CACYBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT3G8

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9HB71

Internal Search Terms :

CACYBP, Clcyclin-binding protein, GIG5, PNAS-107, RP1-102G20.6, S100A6BP, SIP, Clcyclin-binding protein CACYBP, Calcyclin binding protein, GIG 5, Growth inhibiting gene 5, Growth inhibiting gene 5 protein, hCacyBP, MGC87971, PNAS 107, PNAS107, RP1 102G20.6, S100A6 binding protein.,ATGA0316-10ug,ATGA0316-20ug,ATGA0316-50ug,ATGA0316-100ug,ATGA0316-250ug,ATGA0316-500ug,ATGA0316-1mg,ATGA0316-10,ATGA0316-20,ATGA0316-50,ATGA0316-100,ATGA0316-250,ATGA0316-500,ATGA0316-1

more info or order :

NKMAX product webpage