Human ASF1B antibody | NKMAX ATGA0315 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ASF1B antibody

catalog :

ATGA0315

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1D4

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ASF1B antibody

Catalog number (SKU) :

ATGA0315

NCBI :

NP_060624

Description :

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. Delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA. Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress.

Alternative Names :

ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Corpet A., et al. (2011) EMBO J 30:480-493;; Mousson F., et al. (2007) Chromosoma 116:79-93;; Hayashi R., et al. (2007) DNA Cell Biol 126:91-99

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human ASF1B (1-202aa) purified from E. coli

Applications :

ELISA,WB

WB Comment and Pic :

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ASF1B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: ASF1B transfected 293T cell lysate

Reacts With :

Human

Clone Number :

AT1D4

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NVP2

Internal Search Terms :

ASF1B, ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II,ATGA0315-10ug,ATGA0315-20ug,ATGA0315-50ug,ATGA0315-100ug,ATGA0315-250ug,ATGA0315-500ug,ATGA0315-1mg,ATGA0315-10,ATGA0315-20,ATGA0315-50,ATGA0315-100,ATGA0315-250,ATGA0315-500,ATGA0315-1

more info or order :

NKMAX product webpage