Human 14-3-3 gamma antibody | NKMAX ATGA0312 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human 14-3-3 gamma antibody

catalog :

ATGA0312

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT4B9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human 14-3-3 gamma antibody

Catalog number (SKU) :

ATGA0312

NCBI :

NP_036611

Description :

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, Beta, Gamma, Epsilon, Delta, Zeta, Tau and Eta that have been identified in mammals. The 14-3-3 gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Alternative Names :

YWHAG, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma N-terminally processed, protein phosphatase 1, regulatory subunit 170, PPP1R170, 14-3-3γ

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Takagaki Y. and Manley J.L. (1992) J Biol Chem 267: 23471-23474;; Takagaki Y. and Manley J.L. (2000) Mol Cell Biol 20: 1515-1525;; Kleiman F.E. and Manley J.L. (1999) Science 285: 1576-1579

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human YWHAZ ;;Lane 2.: Recombinant Human YWHAB ;;Lane 3.: Recombinant Human YWHAE ;;Lane 4.: Recombinant Human YWHAH ;;Lane 5.: Recombinant Human YWHAG ;;Lane 6.: Recombinant Human SFN ;;Lane 7.: Recombinant Human YWHAQ {ATGA0312-WB2.jpg} The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: A431 cell lysate ;;Lane 3.: K562 cell lysate ;;Lane 4.: NIH3T3 cell lysate {ATGA0312-WB3.jpg}

Reacts With :

Human

Clone Number :

AT4B9

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P61981

Internal Search Terms :

1-4-3-3g, 1433 gamma, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein gamma polypeptide, YWHAG, 14-3-3 gamma,Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, gamma polypeptide 14 3 3 protein gamma, 14 3 3 protein gamma subtype, 14 3 3gamma, 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide, KCIP 1, KCIP1, Protein kinase C inhibitor protein 1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide.,ATGA0312-10ug,ATGA0312-20ug,ATGA0312-50ug,ATGA0312-100ug,ATGA0312-250ug,ATGA0312-500ug,ATGA0312-1mg,ATGA0312-10,ATGA0312-20,ATGA0312-50,ATGA0312-100,ATGA0312-250,ATGA0312-500,ATGA0312-1

more info or order :

NKMAX product webpage