Human IGF-1 antibody | NKMAX ATGA0311 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human IGF-1 antibody

catalog :

ATGA0311

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT6F8

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human IGF-1 antibody

Catalog number (SKU) :

ATGA0311

NCBI :

NP_000609

Description :

Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. IGF-1 has also been referred to as a Sulfation factor and its effects were termed nonsuppressible insulin-like activity (NSILA) in the 1970s. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin, is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7, 649 daltons.

Alternative Names :

IBP1, IGF-1, IGFI, IGF-IA, IGF-IB, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor-1 IGF IA, Mechano growth factor, MGF, Somatomedin C

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hoppener J.W., et al. (1985) Hum Genet 69(2): 157-160;; Jansen M., et al. (1983) Nature 306(5943): 609-611;; Salmon W., et al. (1957) J Lab Clin Med 49(6): 825-836 ;; Keating G.M., (2008) BioDrugs 22(3): 177-188;; Rinderknecht E., et al. (1978) J Biol Chem 253(8): 2769-2776

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human IGF1 (49-118aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF

IF Comment And Pic :

ICC/IF analysis of IGF1 in PC3 cells. The cell was stained with ATGA0311 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The human IGF1 recombinant protein (12.5ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IGF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: IGF1 transfected 293T cell lysate {ATGA0311-WB2.jpg}

Reacts With :

Human

Clone Number :

AT6F8

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P05019

Internal Search Terms :

IBP1, IGF-IB, IGF IB, IGF1, IGF-1, IGFI, IGF-I, IGFIA, IGF-IA, Insulin like growth factor 1, Insulin like growth factor 1 (somatomedin C), Insulin like growth factor IA, Insulin like growth factor IB, Insulin-like growth factor-1, Insulin-like growth factor-1 IGF IA, Mechano growth factor, MGF, Somatomedin C, Somatomedin-C, ATGA0311-10ug, ATGA0311-20ug, ATGA0311-50ug, ATGA0311-100ug, ATGA0311-250ug, ATGA0311-500ug, ATGA0311-1mg, ATGA0311-010, ATGA0311-020, ATGA0311-050, ATGA0311-100, ATGA0311-250, ATGA0311-500, ATGA0311-01M

more info or order :

NKMAX product webpage