Human HIP/ST13 antibody | NKMAX ATGA0309 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human HIP/ST13 antibody

catalog :

ATGA0309

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT5C6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human HIP/ST13 antibody

Catalog number (SKU) :

ATGA0309

NCBI :

NP_003923

Description :

HIP (Hsc70-interacting protein), also known as ST13, is a co-chaperone to the major heat shock proteins, HSP70 and HSP90, and appears in early receptor complexes. Through mutual binding to both HSP70 and HSP90, Hip functions as an adaptor that can integrate HSP70 and HSP90 interactions. Also, Hip has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones.

Alternative Names :

Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Nelson GM, et al., (2004) Mol Endocrinol.18(7): 1620-30;; Johnson BD, et al., (1998) J Biol Chem. 273(6):3679-86

Abs Host :

Mouse

Isotype :

IgG2a kappa

Immunogen :

Recombinant human HIP/ST13 (1-369aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of ST13 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ST13 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0309-IF2.jpg} ICC/IF analysis of ST13 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). {ATGA0309-IF3.jpg}

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ST13 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: HepG2 cell lysate ;;Lane 3.: SW480 cell lysate ;;Lane 4.: Jurkat cell lysate ;;Lane 5.: K562 cell lysate ;;Lane 6.: LnCap cell lysate ;;Lane 7.: HeLa cell lysate ;;Lane 8.: PC3 cell lysate

Reacts With :

Human

Clone Number :

AT5C6

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

FACS Comment and Pic :

Flow cytometry analysis of ST13 in 293T cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). Flow cytometry analysis of ST13 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). {ATGA0309-FC2.jpg}

Antigen Species :

Human

Gene Category :

Heat Shock Proteins

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P50502

Internal Search Terms :

ST13, Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein,ATGA0309-10ug,ATGA0309-20ug,ATGA0309-50ug,ATGA0309-100ug,ATGA0309-250ug,ATGA0309-500ug,ATGA0309-1mg,ATGA0309-10,ATGA0309-20,ATGA0309-50,ATGA0309-100,ATGA0309-250,ATGA0309-500,ATGA0309-1

more info or order :

NKMAX product webpage