Human TBCB antibody | NKMAX ATGA0302 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human TBCB antibody

catalog :

ATGA0302

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1F6

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human TBCB antibody

Catalog number (SKU) :

ATGA0302

NCBI :

NP_001272

Description :

Tubulin folding cofactor B, also known as TBCB, belongs to the Microtubules family. Biosynthesis of functional microtubules involve the participation of several chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), D (TBCD), E (TBCE) and C (TBCC), that act on folding intermediates downstream of the cytosolic chaperon, alternatively named TCP. TBCB is a 244 amino acid cytoplasmic protein containing one CAP-Gly domain and in widely expressed. TBCB is involved in the regulation of tubulin heterodimer dissociation and may function as a negative regulator of axonal growth.

Alternative Names :

Tubulin folding cofactor B, CG22, CKAP1, CKAPI

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kortazar D., et al. (2007) Exp Cell Res. 313:425-436.;; Tian G., et al. (1997) J Cell Biol. 138: 821-832;; Fanarraga ML., et al. (2009) Exp cell Res 315:535-541;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human TBCB (1-244aa) purified from E. coli

Applications :

ELISA,WB,FACS,ICC/IF

IF Comment And Pic :

ICC/IF analysis of TBCB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TBCB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates of Jurkat(40ug) and A549(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCB antibody (1:1500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT1F6

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1500

FACS Comment and Pic :

Flow cytometry analysis of TBCB in HeLa cells. The cell was stained with ATGA0302 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cytoskeleton

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q99426

Internal Search Terms :

TBCB, Tubulin folding cofactor B, CG22, CKAP1, CKAPI,ATGA0302-10ug,ATGA0302-20ug,ATGA0302-50ug,ATGA0302-100ug,ATGA0302-250ug,ATGA0302-500ug,ATGA0302-1mg,ATGA0302-10,ATGA0302-20,ATGA0302-50,ATGA0302-100,ATGA0302-250,ATGA0302-500,ATGA0302-1

more info or order :

NKMAX product webpage