Human CKMT1A antibody | NKMAX ATGA0296 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human CKMT1A antibody

catalog :

ATGA0296

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT17A2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human CKMT1A antibody

Catalog number (SKU) :

ATGA0296

NCBI :

NP_001015001

Description :

CKMT1A, also known as Creatine kinase, mitochondrial 1A, is 417 amino acid protein. Mitochondrial creatine kinase (MtCK) exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK (CKMT1). It is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis.

Alternative Names :

Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Haas. R.C., et al. (1989) J Biol Chem 264(5): 2890-2897 ;; Stachowiak. O., et al. (1998) Mol Cell Biochem 184(1-2): 141-151;; Lipskaya. T.Y. (2001) Biochemistry Mosc 66(10): 1098-1111

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human CKMT1A (40-417aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of CKMT1A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKMT1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKMT1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: HeLa cell lysate

Reacts With :

Human

Clone Number :

AT17A2

Ab Usage :

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

FACS Comment and Pic :

Flow cytometry analysis of CKMT1A in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Apoptosis

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P12532

Internal Search Terms :

CKMT1A, Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK,ATGA0296-10ug,ATGA0296-20ug,ATGA0296-50ug,ATGA0296-100ug,ATGA0296-250ug,ATGA0296-500ug,ATGA0296-1mg,ATGA0296-10,ATGA0296-20,ATGA0296-50,ATGA0296-100,ATGA0296-250,ATGA0296-500,ATGA0296-1

more info or order :

NKMAX product webpage