Human ASNA1 antibody | NKMAX ATGA0293 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human ASNA1 antibody

catalog :

ATGA0293

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2A1

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human ASNA1 antibody

Catalog number (SKU) :

ATGA0293

NCBI :

NP_004308

Description :

ASNA1, also as known as ARSA and TRC40, is the human homolog of the bacterial arsA, a member of the ATPase superfamily. ArsA and ArsB have been postulated to form a membrane complex which functions as an anion-translocating. It recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ArsA hydrolyses ATP in the presence of its anionic substrate antimonite and produces resistance to arsenite and antimonite. The active form of ArsA is a homodimer with four nucleotide binding sites, two from each monomer.

Alternative Names :

ATPase ASNA1, ARSA-I, ARSA1, ASNA-I, GET3, hASNA-I, TRC40

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ota T and Suzuki Y. (2004) Nat genet 36(1): 40-5.;; Kurdi-Haidar B. (1996) Genomics 36(3): 486-91.;; Stefanovic S. (2007) Cell 128(6): 1147-59.;;

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human ASNA1 (1-348aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of ASNA1 in HeLa cells. The cell was stained with ATGA0293 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ASNA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: HeLa cell lysate ;;Lane 2.: 293T cell lysate ;; Lane 3.: MCF7 cell lysate

Reacts With :

Human

Clone Number :

AT2A1

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of ASNA1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Metabolism

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O43681

Internal Search Terms :

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more info or order :

NKMAX product webpage