Human DNAL1 antibody | NKMAX ATGA0287 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human DNAL1 antibody

catalog :

ATGA0287

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT29E4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human DNAL1 antibody

Catalog number (SKU) :

ATGA0287

NCBI :

NP_113615

Description :

DNAL1, also known as dynein light chain 1 axonemal, is component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. DNAL1 is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Homozygous mutations in the DNAL1 gene are associated with primary ciliary dyskinesia 16 (CILD16) which is a disorder characterized by abnormalities of motile cilia.

Alternative Names :

Dynein light chain 1 axonemal, Dynein light chain 1, axonemal, C14orf168, CILD16

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Mazor. M., et al. (2011) Am J Hum Genet 88: 599-607.;; Horvath. J., et al. (2005) Am J Respir Cell Mol Biol 33: 41-47.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human DNAL1 (1-190aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF,FACS

IF Comment And Pic :

ICC/IF analysis of DNAL1 in HeLa cells. The cell was stained with ATGA0287 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of DNAL1 in A549 cells. The cell was stained with ATGA0287 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0287-IF2.jpg}

WB Comment and Pic :

The cell lysates of HeLa, HepG2 and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DNAL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Reacts With :

Human

Clone Number :

AT29E4

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

FACS Comment and Pic :

Flow cytometry analysis of DNAL1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Antigen Species :

Human

Gene Category :

Signal Transduction

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q4LDG9

Internal Search Terms :

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more info or order :

NKMAX product webpage